6HL8

Crystal Structure of the CsiD Glutarate Hydroxylase in complex with Glutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 4 21 2
Unit cell lengths	120.800, 120.800, 136.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.699 - 2.400
R-factor	0.1773
Rwork	0.175
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.923
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.559	2.380
High resolution limit [Å]	2.340	2.340
Number of reflections	43225
<I/σ(I)>	8.12
Completeness [%]	99.9
Redundancy	7.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.08 M Sodium chloride, 0.012 M Potassium chloride, 0.02 M Magnesium chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate pH 6.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride.