6HL6

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and iASPP(670-693)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 41 21 2
Unit cell lengths	86.084, 86.084, 148.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.507 - 1.970
R-factor	0.1724
Rwork	0.171
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.011
RMSD bond angle	0.956
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.510	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.074	1.980
Number of reflections	40372	1980
<I/σ(I)>	14081	1.3
Completeness [%]	100.0	98.9
Redundancy	8.48	8.7
CC(1/2)	0.988	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	273	0.1 M Hepes pH = 7.5, 1.6 M ammonium sulfate, 3.5% PEG400