6HL5
Factor Inhibiting HIF (FIH) in complex with zinc, NOG and ASPP1(932-954)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.198, 86.198, 147.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.310 - 1.980 |
| R-factor | 0.1829 |
| Rwork | 0.182 |
| R-free | 0.20130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h2k |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.150 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.310 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.080 | 2.350 |
| Number of reflections | 39456 | 2880 |
| <I/σ(I)> | 17 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.9 | 17.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Hepes pH = 7.5, 1.2 M ammonium sulfate, 4.5% PEG400 |
