6HL2
wild-type NuoEF from Aquifex aeolicus - oxidized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.653080 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.190, 116.438, 189.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.480 - 1.950 |
| R-factor | 0.1711 |
| Rwork | 0.170 |
| R-free | 0.19500 |
| Structure solution method | SAD |
| Starting model (for MR) | INHOUSE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | Aimless ((version 0.5.12)) |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.440 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.162 | 1.561 |
| Number of reflections | 102591 | |
| <I/σ(I)> | 12.9 | 1.5 |
| Completeness [%] | 99.9 | 97.8 |
| Redundancy | 15.9 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 292 | 0.1 M Tris/HCl, 1.0 M sodium citrate |
