6HKV
Trichodysplasia spinulosa-associated polyomavirus (TSPyV) VP1 in complex with sialylated precision glycooligomers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-11-27 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 138.400, 145.705, 149.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.950 - 1.750 |
R-factor | 0.1761 |
Rwork | 0.176 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u60 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.302 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.950 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmeas | 0.110 | 1.780 |
Number of reflections | 302337 | 29947 |
<I/σ(I)> | 15.72 | 1.29 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 8.7 | 9 |
CC(1/2) | 0.999 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, sodium malonate pH 5.0 |