6HKR
Human Cellular Retinoic Acid Binding Protein II (CRABPII) with bound synthetic retinoid DC271.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.689, 47.479, 77.733 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.550 - 1.500 |
| R-factor | 0.14462 |
| Rwork | 0.143 |
| R-free | 0.17548 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fr3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.903 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.550 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 27170 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG5000, TRIS HCL, Sodium Acetate |
