6HKE
MatC (Rpa3494) from Rhodopseudomonas palustris with bound malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.800, 205.750, 47.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.540 - 2.110 |
| R-factor | 0.19328 |
| Rwork | 0.191 |
| R-free | 0.24814 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oei |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.540 | 2.170 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.046 | 0.591 |
| Number of reflections | 41492 | 2471 |
| <I/σ(I)> | 12.4 | 1.6 |
| Completeness [%] | 88.9 | 65.3 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 290 | Tris 0.1 M pH 8.5, MgCl2 0.2 M, PEG 8000 20% (w/v) |
