6HJK

Crystal Structure of Aurora-A L210C catalytic domain in complex with ASDO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92818
Spacegroup name	P 32 2 1
Unit cell lengths	88.769, 88.769, 77.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.876 - 2.400
R-factor	0.2189
Rwork	0.217
R-free	0.25230
RMSD bond length	0.010
RMSD bond angle	1.221
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.880	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	14112	1373
<I/σ(I)>	23.07	4.63
Completeness [%]	100.0	100
Redundancy	2	2
CC(1/2)	1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium sulfate, 0.1 M tri-sodium citrate pH 5.6, 25 % w/v polyethylene glycol 4000