6HJI
Xray structure of GLIC in complex with crotonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2016-07-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.943, 133.242, 159.880 |
| Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.800 |
| R-factor | 0.1896 |
| Rwork | 0.189 |
| R-free | 0.20430 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 49.000 |
| High resolution limit [Å] | 2.800 | 2.900 |
| Number of reflections | 91038 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Na-crotonate pH4 400 mM Na-Isothiocyanate 12-15% PEG4000 2% DMSO 15% glycerol |
