6HJB
Xray structure of GLIC in complex with malonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2015-04-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.099, 133.366, 158.987 |
| Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
| Resolution | 49.000 - 3.000 |
| R-factor | 0.1827 |
| Rwork | 0.182 |
| R-free | 0.19900 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.300 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 312634 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Na-malonate pH4 400 mM Na-Isothiocyanate 12-15% PEG4000 2% DMSO 16% Glycerol |
