6HIP
Structure of SPF45 UHM bound to HIV-1 Rev ULM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.210, 63.680, 67.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.490 - 1.200 |
| R-factor | 0.15022 |
| Rwork | 0.149 |
| R-free | 0.17502 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pe8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.490 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmeas | 0.170 | 0.647 |
| Number of reflections | 64821 | 9278 |
| <I/σ(I)> | 8.18 | |
| Completeness [%] | 98.5 | 91.3 |
| Redundancy | 7.82 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22.5 % (w/v) PEG 3350, 0.1 M HEPES pH 6.0, 0.2 M sodium acetate |
