6HHN
Crystal structure of L-rhamnose mutarotase FA22100 from Formosa agariphila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979140 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.913, 70.913, 54.407 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.166 - 1.470 |
| R-factor | 0.1491 |
| Rwork | 0.148 |
| R-free | 0.17210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qlx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.889 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.170 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.032 | 0.295 |
| Rmeas | 0.034 | 0.315 |
| Rpim | 0.012 | 0.107 |
| Number of reflections | 24225 | 3383 |
| <I/σ(I)> | 32.3 | 6.6 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 9.1 | 8.5 |
| CC(1/2) | 0.999 | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Sodium acetate pH4.6 4.3 M Sodium formate |
