6HH4
ADP-ribosylserine hydrolase ARH3 of Latimeria chalumnae in complex with ADP-ribosyl-L-arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.145, 96.642, 105.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.390 - 1.660 |
| R-factor | 0.16965 |
| Rwork | 0.169 |
| R-free | 0.18994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2foz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.390 | 1.700 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.089 | 1.524 |
| Rmeas | 0.101 | 1.733 |
| Rpim | 0.047 | 0.809 |
| Number of reflections | 82067 | 5982 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 8.4 |
| CC(1/2) | 0.997 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 200 MM AMMONIUM ACETATE, 25 % (W/V), PEG4000, 100 MM CITRATE PH 5.6 |
