6HF2
The structure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.812, 68.376, 79.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.787 - 1.690 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.20630 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.790 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Number of reflections | 40664 | |
<I/σ(I)> | 18.23 | |
Completeness [%] | 98.5 | |
Redundancy | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1 M bis-tris, 20% PEG4000, 0.2 M NaCl, 0.3 mM CaCl2 |