6HF1
Mutant oxidoreductase fragment of mouse QSOX1 in complex with an antibody Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.539, 112.715, 193.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.886 - 1.940 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d93 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.000 | 2.020 |
| High resolution limit [Å] | 1.937 | 1.940 |
| Number of reflections | 104667 | 8728 |
| <I/σ(I)> | 13.7 | 1.6 |
| Completeness [%] | 97.6 | 82.5 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M sodium citrate, pH 6.0 16% w/v PEG |
