6HEP

Crystal structure of human 14-3-3 beta in complex with CFTR R-domain peptide pS753-pS768

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-11-26
Detector	PSI PILATUS 6M
Wavelength(s)	1.07027
Spacegroup name	P 21 21 21
Unit cell lengths	70.619, 111.599, 130.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.375 - 1.860
R-factor	0.2035
Rwork	0.201
R-free	0.25100
RMSD bond length	0.007
RMSD bond angle	0.790
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((dev_3139: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.620	10.000
High resolution limit [Å]	1.340	1.680
Number of reflections	231938
<I/σ(I)>	6.7
Completeness [%]	99.7
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	278	0.1M Tris, 25% v/v PEG350 MME