6HEL
Structure of human USP25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | I 4 |
| Unit cell lengths | 139.184, 139.184, 190.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.166 - 2.941 |
| R-factor | 0.2551 |
| Rwork | 0.254 |
| R-free | 0.27750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6HEH and 6HEK |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.134 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.170 | 3.390 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.052 | 1.430 |
| Rmeas | 0.058 | 1.570 |
| Number of reflections | 20988 | 1050 |
| <I/σ(I)> | 15.9 | 1.2 |
| Completeness [%] | 93.4 | 70.1 |
| Redundancy | 5.3 | 5.9 |
| CC(1/2) | 0.999 | 0.501 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 12% (w/v) PEG 3350, 167 mM magnesium acetate |
