6HEH

Structure of the catalytic domain of USP28 (insertion deleted)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	I 41 3 2
Unit cell lengths	189.152, 189.152, 189.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.550 - 2.260
R-factor	0.1966
Rwork	0.196
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hei
RMSD bond length	0.003
RMSD bond angle	0.600
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.550	2.340
High resolution limit [Å]	2.260	2.260
Rmerge	0.065	0.699
Rmeas	0.068	0.752
Number of reflections	27219	2698
<I/σ(I)>	19.8	2.7
Completeness [%]	99.8	99.2
Redundancy	8.6	7.5
CC(1/2)	0.999	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	291	12% (w/v) PEG 8000, 100 mM sodium chloride, 200 mM lithium sulfate, 100 mM MES pH 6.6