6HDS
Crystal Structure of apo short afifavidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.991, 64.952, 68.114 |
| Unit cell angles | 90.00, 100.07, 90.00 |
Refinement procedure
| Resolution | 46.700 - 1.740 |
| R-factor | 0.18679 |
| Rwork | 0.184 |
| R-free | 0.23066 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.559 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.060 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 35450 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 95.7 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 1.3-1.5M Ammonium Sulfate, 0.1M Acetate pH 5.0 |
