6HDB

Crystal structure of the potassium channel MtTMEM175 with zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.24610
Spacegroup name	P 4 21 2
Unit cell lengths	131.920, 131.920, 132.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.100 - 2.900
R-factor	0.276
Rwork	0.275
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf 5jqh
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.640	3.004
High resolution limit [Å]	2.900	2.900
Rmeas	0.177	14.320
Rpim	0.026	2.230
Number of reflections	26575	2588
<I/σ(I)>	10.2
Completeness [%]	99.9	99.8
Redundancy	27	27.1
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28- 30% PEG400