6HDA

Crystal structure of the potassium channel MtTMEM175 with cesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	2.00690
Spacegroup name	P 4 21 2
Unit cell lengths	131.680, 131.680, 131.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.210 - 3.800
R-factor	0.28
Rwork	0.280
R-free	0.33700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf 5jqh
RMSD bond length	0.009
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.500	3.940
High resolution limit [Å]	3.800	3.800
Rmeas	0.248	7.760
Rpim	0.019	0.417
Number of reflections	11875	1153
<I/σ(I)>	10.2	1
Completeness [%]	99.9	100
Redundancy	40.7	40
CC(1/2)	1.000	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28- 30% PEG400