6HCE

Crystal structure of chicken riboflavin binding protein in "Apo" form at 2.5 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION SUPERNOVA
Temperature [K]	120
Detector technology	CCD
Collection date	2015-02-20
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	110.008, 110.008, 71.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.890 - 2.500
R-factor	0.18447
Rwork	0.181
R-free	0.23076
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmz
RMSD bond length	0.007
RMSD bond angle	1.269
Data reduction software	CrysalisPro
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.440	15.440	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge	0.135	0.031	0.567
Rmeas	0.164	0.040	0.719
Rpim	0.093	0.025	0.434
Number of reflections	17468	464	2546
<I/σ(I)>	8
Completeness [%]	99.1	78.1	99.7
Redundancy	3	1.9	2.6
CC(1/2)	0.986	0.998	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.5 M Ammonium sulfate, 100 mM HEPES; pH 7.5