6HC5
The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with audouine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9196 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 55.826, 55.826, 133.363 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.913 - 1.413 |
| R-factor | 0.1765 |
| Rwork | 0.175 |
| R-free | 0.19890 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.801 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3228: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.913 | 1.437 |
| High resolution limit [Å] | 1.413 | 1.413 |
| Rmerge | 0.047 | 1.920 |
| Rpim | 0.021 | 0.813 |
| Number of reflections | 28892 | 775 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 6.3 | 6.5 |
| CC(1/2) | 1.000 | 0.428 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.01 M Magnesium Acetate, 0.05 M MES pH 5.0, 2.5 M Ammonium Sulfate |
