6HBZ

Bdellovibrio bacteriovorus DgcB Full-length

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 65 2 2
Unit cell lengths	176.776, 176.776, 112.117
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	153.090 - 1.790
R-factor	0.1811
Rwork	0.179
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.593
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	153.090	90.450	1.820
High resolution limit [Å]	1.790	9.780	1.790
Rmerge	0.121	0.050	1.958
Rmeas	0.123	0.051	1.985
Rpim	0.020	0.009	0.325
Number of reflections	97476	724	4737
<I/σ(I)>	20.4
Completeness [%]	100.0	99.9	99.6
Redundancy	37	33.4	36.9
CC(1/2)	0.998	0.998	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2M KBr, 0.1M sodium acetate, 15% PEG 4000