6HBX

The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with ethylguanidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9197
Spacegroup name	H 3 2
Unit cell lengths	56.070, 56.070, 134.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.035 - 1.540
R-factor	0.1917
Rwork	0.190
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.380
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3228: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.035	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.053	1.436
Rpim	0.026	0.696
Number of reflections	22399	584
<I/σ(I)>	17.3
Completeness [%]	97.7	96.5
Redundancy	4.9	5.1
CC(1/2)	1.000	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate