6HBM

Crystal structure of MSMEG_1712 from Mycobacterium smegmatis in complex with alpha-L-arabinofuranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	119.500, 119.500, 234.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.950 - 2.760
R-factor	0.221
Rwork	0.218
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hb0
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.500	3.190
High resolution limit [Å]	2.750	2.750
Number of reflections	63068
<I/σ(I)>	17.5
Completeness [%]	92.9
Redundancy	25.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris, pH 8.0 21 % PEG 400 0.325 M NaOAc 0.1 M NaCl