6HBD

Crystal structure of MSMEG_1712 from Mycobacterium smegmatis in complex with Beta-D-Galactofuranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	118.300, 118.300, 231.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.310 - 2.440
R-factor	0.229
Rwork	0.227
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hb0
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.310	2.767
High resolution limit [Å]	2.440	2.445
Number of reflections	118103
<I/σ(I)>	20.2
Completeness [%]	94.7
Redundancy	27.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8.0 21 % PEG 400 0.325 M NaOAc 0.1 M NaCl