6HAU

KSHV PAN RNA Mta-response element fragment complexed with the globular domain of herpesvirus saimiri ORF57

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 21 21 21
Unit cell lengths	58.657, 105.261, 118.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.710 - 1.860
R-factor	0.16
Rwork	0.158
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo HVS ORF57
RMSD bond length	0.008
RMSD bond angle	0.924
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.710	1.925
High resolution limit [Å]	1.858	1.858
Rmerge	0.073	0.725
Rmeas	0.079	0.777
Number of reflections	62577	6135
<I/σ(I)>	18.23	2.67
Completeness [%]	99.9	99.79
Redundancy	7.5	7.7
CC(1/2)	0.999	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.06 M divalents (0.3M Magnesium chloride hexahydrate; 0.3M Calcium chloride dehydrate), 0.1 M (Imidazole, MES) Buffer System pH 6.5, 50 % v/v GOL_P4K (Morpheus HT96 A3 Molecular Dimensions)