6HAO
MamM CTD H264E - Cadmium form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.661, 53.661, 58.074 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.470 - 2.400 |
| R-factor | 0.24499 |
| Rwork | 0.238 |
| R-free | 0.31440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.470 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.169 | |
| Number of reflections | 3988 | |
| <I/σ(I)> | 11.9 | 2.3 |
| Completeness [%] | 98.6 | 88.4 |
| Redundancy | 9.2 | 4.7 |
| CC(1/2) | 0.994 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M MgCl2, 0.1M BIS-TRIS pH=6.5, 25% PEG 3350, 1.7mM CdCl2 |
