6HAK
Crystal structure of HIV-1 reverse transcriptase (RT) in complex with a double stranded RNA represents the RT transcription initiation complex prior to nucleotide incorporation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 175.240, 175.240, 225.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.620 - 3.950 |
| R-factor | 0.296 |
| Rwork | 0.293 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5txl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.987 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.620 | 4.160 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.236 | 2.446 |
| Number of reflections | 31549 | |
| <I/σ(I)> | 6.7 | 0.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.8 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE |
