6H9J

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPV3 (229-255)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	86.400, 86.400, 149.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.635 - 1.830
R-factor	0.1668
Rwork	0.166
R-free	0.18680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.013
RMSD bond angle	1.065
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.635	1.860
High resolution limit [Å]	1.830	1.830
Rmerge	0.105	2.854
Rmeas	0.108	2.928
Rpim	0.025	0.649
Number of reflections	50768	2483
<I/σ(I)>	13.74	1.2
Completeness [%]	100.0	100
Redundancy	19.4	20.1
CC(1/2)	0.998	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Hepes pH = 7.5, 1.8 M ammonium sulfate, 4.5% PEG400