6H9I
Csf5, CRISPR-Cas type IV Cas6 crRNA endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.850, 84.237, 71.472 |
| Unit cell angles | 90.00, 100.35, 90.00 |
Refinement procedure
| Resolution | 43.762 - 2.290 |
| R-factor | 0.2247 |
| Rwork | 0.223 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h9h |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.792 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.762 | 2.370 |
| High resolution limit [Å] | 2.288 | 2.290 |
| Rmerge | 0.074 | |
| Rmeas | 0.090 | |
| Rpim | 0.050 | |
| Number of reflections | 32620 | |
| <I/σ(I)> | 10.83 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M di-sodium tartrate, 20% w/v PEG3350 |
