6H85
MamM CTD H264E-E289H - Cadmium form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.231, 94.249, 53.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.120 - 2.000 |
| R-factor | 0.18932 |
| Rwork | 0.186 |
| R-free | 0.22012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.100 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | |
| Number of reflections | 6470 | |
| <I/σ(I)> | 29.1 | 8.9 |
| Completeness [%] | 99.6 | 95.1 |
| Redundancy | 12.4 | 10.2 |
| CC(1/2) | 0.999 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M AmSu, 0.1M BIS-TRIS pH=5.5, 25% PEG 3350, 1.7mM CdCl2 |
