6H7X

First X-ray structure of full-length human RuvB-Like 2.

Replaces:  5N7R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	273.15
Detector technology	CCD
Collection date	2015-07-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97620
Spacegroup name	P 6
Unit cell lengths	123.023, 123.023, 60.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.269 - 2.892
R-factor	0.2065
Rwork	0.205
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c9o
RMSD bond length	0.002
RMSD bond angle	0.424
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2992: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.859	2.940
High resolution limit [Å]	2.892	2.892
Rmerge	0.111
Rmeas	0.129
Rpim	0.063
Number of reflections	11583
<I/σ(I)>	11.3
Completeness [%]	97.0
Redundancy	3.9
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	303.15	PEG 3350, Magnesium Chloride