6H7M

ACTIVATED TURKEY BETA1 ADRENOCEPTOR WITH BOUND PARTIAL AGONIST SALBUTAMOL AND NANOBODY Nb6B9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	116.640, 121.500, 130.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.770 - 2.760
R-factor	0.26571
Rwork	0.265
R-free	0.28515
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h6x 3p0g
RMSD bond length	0.008
RMSD bond angle	1.299
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0166)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.770	2.680
High resolution limit [Å]	2.610	2.610
Number of reflections	56733
<I/σ(I)>	5
Completeness [%]	99.0
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M Hepes-NaOH pH7.5 and 21-24% PEG1500