6H7L

ACTIVATED TURKEY BETA1 ADRENOCEPTOR WITH BOUND PARTIAL AGONIST DOBUTAMINE AND NANOBODY Nb6B9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.919
Spacegroup name	P 21 21 21
Unit cell lengths	116.513, 119.699, 129.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.080 - 2.700
R-factor	0.24267
Rwork	0.241
R-free	0.27826
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h6x 3p0g
RMSD bond length	0.009
RMSD bond angle	1.286
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0174)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.080	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	50091
<I/σ(I)>	4.9
Completeness [%]	99.4
Redundancy	15.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M Hepes-NaOH pH7.5 and 21-24% PEG1500