6H7E
GEF regulatory domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 298.15 |
| Detector technology | PIXEL |
| Collection date | 2017-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 41.698, 105.617, 160.556 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 88.237 - 2.300 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.240 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 180358 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 20 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS propane; pH 6.5 200 mM Sodium Potassium phosphate |
