6H7B

Structure of Leishmania PABP1 (domain J) complexed with a peptide containing the PAM2 motif of eIF4E4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 65
Unit cell lengths	83.330, 83.330, 58.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.083 - 1.890
R-factor	0.1989
Rwork	0.197
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h7a
RMSD bond length	0.004
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.100	1.958
High resolution limit [Å]	1.890	1.890
Rmerge	0.057	1.500
Rmeas	0.060	1.607
Rpim	0.019	0.574
Number of reflections	18507	1829
<I/σ(I)>	18.54
Completeness [%]	99.9	99.84
Redundancy	9.8	7.8
CC(1/2)	0.999	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2M ammonium sulphate; 0.1M MES; 20% PEG 8000