6H6Z
GI.1 human norovirus protruding domain in complex with Nano-62
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.240, 89.640, 61.920 |
| Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
| Resolution | 46.672 - 2.085 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.21670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zl5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.071 | 0.554 |
| Number of reflections | 51659 | |
| <I/σ(I)> | 11.2 | 1.7 |
| Completeness [%] | 96.9 | 82.8 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium citrate (pH 5.5) and 20% (w/v) PEG3000 |
