6H6P
UbiJ-SCP2 Ubiquinone synthesis protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.690, 94.550, 115.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.280 - 2.500 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h6n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.280 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 18749 | |
| <I/σ(I)> | 9.13 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 225 mM Calcium Chloride, 100 mM MMT buffer pH 9, 11% w/v PEG 8000 |
