6H6H
Crystal structures of the murine class I major histocompatibility complex H-2Dbm13 in complex with adenovirus-derived peptide Ad10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.410, 70.270, 87.050 |
| Unit cell angles | 83.98, 86.90, 81.75 |
Refinement procedure
| Resolution | 19.940 - 2.400 |
| R-factor | 0.2026 |
| Rwork | 0.200 |
| R-free | 0.24869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n5a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.443 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.570 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 42580 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.8M ammonium sulphate, 0.1M Tris |
