6H65
Crystal structure of the branched-chain-amino-acid aminotransferase from Haliangium ochraceum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.080, 164.930, 254.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.640 - 2.350 |
| R-factor | 0.20108 |
| Rwork | 0.199 |
| R-free | 0.23477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.088 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmeas | 0.011 | 0.901 |
| Number of reflections | 80822 | |
| <I/σ(I)> | 8.28 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | MES 0.1 M pH 6.0, CaCl2 0.2M, MPD 50% |
