6H5N
Plasmodium falciparum Pfs48/45 C-terminal domain bound to monoclonal antibody 85RF45.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 59.690, 165.390, 189.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.980 - 3.230 |
| R-factor | 0.262 |
| Rwork | 0.261 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k2u |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.850 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.980 | 3.290 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Number of reflections | 30979 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M ammonium dihydrogen phosphate, 0.1M Tris 8.5 Buffer, 50 % v/v 2-methyl-2,4-pentanediol |
