6H57
Crystal structure of S. cerevisiae DEAH-box RNA helicase Dhr1, essential for small ribosomal subunit biogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-09-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.314, 115.800, 157.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.830 - 2.300 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kx2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.830 | 48.830 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.111 | 0.023 | 0.995 |
| Rmeas | 0.131 | 0.027 | 1.176 |
| Rpim | 0.067 | 0.014 | 0.611 |
| Total number of observations | 157599 | ||
| Number of reflections | 45887 | 839 | 4508 |
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 97.1 | 91.4 | 98.6 |
| Redundancy | 3.4 | 3.2 | 3.3 |
| CC(1/2) | 0.995 | 0.999 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 0.2 M MgCl2; 0.1 M Tris pH 8.5 and 20% (w/v) PEG 8000 |
