6H56
Effector domain of Pseudomonas aeruginosa VgrG2b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 |
| Unit cell lengths | 78.310, 78.310, 115.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.930 - 3.200 |
| R-factor | 0.2238 |
| Rwork | 0.223 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.930 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.420 | |
| Rmeas | 0.090 | 0.757 |
| Number of reflections | 12896 | |
| <I/σ(I)> | 10.9 | 2.3 |
| Completeness [%] | 98.5 | 97.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M bis-tris, 45% polypropylene glycol P400 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M bis-tris, 45% polypropylene glycol P400 |
