6H4B

A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	I 2 3
Unit cell lengths	122.896, 122.896, 122.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.448 - 2.902
R-factor	0.2587
Rwork	0.254
R-free	0.30520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Chain A 5mil chain B 6H48
RMSD bond length	0.009
RMSD bond angle	1.495
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.900	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.099	1.038
Rpim		0.183
Number of reflections	7002	1110
<I/σ(I)>	23.7	4.8
Completeness [%]	100.0	100
Redundancy	33.3	33.4
CC(1/2)	1.000	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	10%PEG4000, 10%isopropanol, 0.1MNa-Citrate