6H3S

Schmallenberg Virus Glycoprotein Gc Head/Stalk Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.919080
Spacegroup name	P 41 21 2
Unit cell lengths	95.226, 95.226, 204.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.613 - 2.023
R-factor	0.2113
Rwork	0.209
R-free	0.24040
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.561
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.930	2.280
High resolution limit [Å]	2.010	2.010
Rmerge	0.100	1.910
Rmeas	0.100	1.970
Rpim	0.020	0.490
Number of reflections	38793	1940
<I/σ(I)>	20.6	1.7
Completeness [%]	60.9	9.8
Redundancy	17.6	15.6
CC(1/2)	1.000	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.5 uL of 12.3 mg/mL EndoH/EndoD-deglycosylated SBV Gc(465-874) in 17.6 mM Tris-Cl pH 8.0, 132 mM NaCl, 0.5 M ammonium sulfate were added to 0.75 uL of reservoir solution containing 0.1 M MES pH6.5, 5%w/v PEG 400, 2 M ammonium sulfate