6H3R
Crystal structure of Smad2 without exon -MH1 bound to the CAGAC site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.140, 80.140, 143.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.800 - 2.750 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ozj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.760 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmeas | 0.092 | 0.127 |
| Number of reflections | 12777 | 154 |
| <I/σ(I)> | 14.85 | 1.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.3 | 9.9 |
| CC(1/2) | 0.994 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES 6.5 30% PEG300 |
