6H3A

Crystal structure of the KAP1 RBCC domain in complex with the SMARCAD1 CUE1 domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976
Spacegroup name	I 2 3
Unit cell lengths	299.914, 299.914, 299.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.978 - 5.505
R-factor	0.2805
Rwork	0.279
R-free	0.30260
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.832
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.100	6.150
High resolution limit [Å]	5.500	5.500
Rmerge	0.155	1.250
Rpim	0.040	0.290
Number of reflections	14734	3240
<I/σ(I)>	11.5	2.6
Completeness [%]	100.0	100
Redundancy	19.7	19.5
CC(1/2)	0.999	0.160

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.2 M Sodium Malonate, 0.5 % Jeffamine ED-2003 and 0.1 M HEPES pH 7.0