6H3A
Crystal structure of the KAP1 RBCC domain in complex with the SMARCAD1 CUE1 domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.976 |
Spacegroup name | I 2 3 |
Unit cell lengths | 299.914, 299.914, 299.914 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.978 - 5.505 |
R-factor | 0.2805 |
Rwork | 0.279 |
R-free | 0.30260 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.832 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.100 | 6.150 |
High resolution limit [Å] | 5.500 | 5.500 |
Rmerge | 0.155 | 1.250 |
Rpim | 0.040 | 0.290 |
Number of reflections | 14734 | 3240 |
<I/σ(I)> | 11.5 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.7 | 19.5 |
CC(1/2) | 0.999 | 0.160 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.2 M Sodium Malonate, 0.5 % Jeffamine ED-2003 and 0.1 M HEPES pH 7.0 |