6H2Y

human Fab 1E6 bound to fHbp variant 3 from Neisseria meningitidis serogroup B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.983
Spacegroup name	P 2 21 21
Unit cell lengths	42.979, 65.279, 278.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.561 - 2.650
R-factor	0.2083
Rwork	0.205
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.046
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.280	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.159	0.565
Number of reflections	23201	3370
<I/σ(I)>	6.7	2.4
Completeness [%]	97.7	98.7
Redundancy	4	4.2
CC(1/2)	0.987	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	Molecular Dimensions Morpheus HT-96 screen, well D9